Biological Activity of Root Extract Decalepis hamiltonii (Wight & Arn) against Three Mosquito Vectors and Their Non-Toxicity against the Mosquito Predators

Bioactive molecules of plant origin play a significant role as defensive agents in different insect species. Chemical compounds medicinal plants have been an exciting alternative to standard methods controlling mosquito larvae. The present study evaluates the solvent extracts D. hamiltonii for toxicity against three Bioassay revealed that effect methanol increased larval mortality with increasing concentration. highest was observed Culex quinquefasciatus 98.33%, followed by 95 and 90% Aedes aegypti Anopheles stephensi, at 24 h exposure. GC-MS analysis extract showed six major peak compounds. They are benzaldehyde, 2-hydroxy-4-methoxy-(10.35%), dodecanoic acid (11.02%), n-hexadecanoic (21.05%), linoleic methyl ester (14.20%), oleic (21.04%), octadecanoic (22.21%). level α β Carboxylesterases gets significantly decreased post-treatment dose-dependent manner.In contrast, glutathione S-transferase (GST) cytochrome-P450 (CYP450) levels get up-lifted steadily when dosage increased. ratio GST has drastically proclaimed Ae. 0.702 mg/m Lin parallel Cx. quiquefasciatus (0.656 mg/mL) An. stephensi (0.812 mg/mL). Cytochrome P450 activity increase sub-lethal hamiltonii. Correspondingly, non-target screening aquatic predators reveals crude root their derivatives ecologically safe less toxic. Overall, research highlights chemical characterization hamiltonii, insecticidal medically challenging pests, delivers safe, target specific bio-active suitable substitute pesticides.